Related antibody reaction rule in Elisa kit experiment - Database & Sql Blog Articles

**The Reaction Law of Related Antibodies in ELISA Kit Experiments** ELISA, or Enzyme-Linked Immunosorbent Assay, is a widely used technique in immunology for detecting and quantifying specific antibodies or antigens in a sample. The basic principle of ELISA involves the immobilization of an antigen or antibody on a solid surface, such as a microplate, and the use of an enzyme-labeled counterpart to detect the presence of target molecules. During the experiment, the antigen or antibody bound to the solid support retains its biological activity, as does the enzyme-labeled molecule. When the test sample is introduced, it reacts with the immobilized antigen or antibody, forming a complex. After washing, unbound substances are removed, and the enzyme-labeled reagent is added. This leads to the formation of a complex that includes the enzyme, which then catalyzes a reaction with a substrate, producing a measurable color change. The intensity of the color is directly proportional to the concentration of the target substance in the sample, allowing for both qualitative and quantitative analysis. Due to the high catalytic efficiency of enzymes, even small amounts of the target can be detected, making ELISA a highly sensitive method. In terms of antibody production, there are three main stages: 1. **Primary Response**: When an antigen first enters the body, it takes time for the immune system to recognize and produce antibodies. The amount of antibodies generated during this phase is relatively low and they remain in the body for a short period. 2. **Secondary Response**: Upon re-exposure to the same antigen, the immune system responds more rapidly and produces a much larger quantity of antibodies—often several times higher than the primary response. These antibodies stay in the body longer, providing a stronger and faster immune defense. 3. **Memory Response**: After some time, the initial antibodies may decrease. However, if the same antigen is encountered again, the memory cells quickly generate a new wave of antibodies. This is known as a specific recall response when the antigen is the same as before, or a non-specific recall response if the antigen is different. The latter typically results in a temporary increase in antibody levels. Our company provides high-quality, cost-effective ELISA kits, available in 96T or 48T formats. If you need assistance or have any questions, feel free to contact us via phone, QQ, or mobile. We are here to help!

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