Related antibody reaction rule in Elisa kit experiment - Database & Sql Blog Articles

**The Reaction Law of Related Antibodies in ELISA Kit Experiments** ELISA, or Enzyme-Linked Immunosorbent Assay, is a widely used technique in immunology for detecting and quantifying specific antibodies or antigens in a sample. The basic principle involves the immobilization of an antigen or antibody on a solid surface, such as a microplate, and the use of an enzyme-labeled counterpart to detect the presence of the target molecule. When the antigen or antibody is fixed on the solid support, it retains its immunological activity. Similarly, the enzyme-labeled reagent also maintains both its immunological and enzymatic properties. During the experiment, the test sample is added, allowing any specific antibodies or antigens present to bind to the immobilized molecules on the plate. After washing away unbound substances, the enzyme-labeled reagent is introduced, forming a complex with the bound target. The amount of enzyme attached to the solid phase is directly proportional to the concentration of the analyte in the original sample. When a substrate is added, the enzyme catalyzes a reaction that produces a measurable color change. This color intensity is then used to determine the quantity of the target substance in the sample, making ELISA a highly sensitive and reliable method. Due to the high catalytic efficiency of enzymes, even small amounts of target molecules can be detected, effectively amplifying the immune response signal. This makes ELISA ideal for various applications, including diagnostic testing, drug development, and research. **Key Stages of Antibody Production in ELISA:** 1. **Primary Reaction:** When an antigen is first introduced into the body, the immune system takes some time to produce antibodies. The initial antibody production is limited in quantity and remains in the body for a short period. 2. **Secondary Reaction:** Upon re-exposure to the same antigen, the immune system responds more rapidly. The pre-existing antibodies quickly bind to the antigen, leading to a rapid and significant increase in antibody titer—often several times higher than the primary response. These antibodies remain in the body for a longer duration. 3. **Recall Reaction:** If the antigen is encountered again after the initial antibody levels have declined, the immune system can "remember" the previous exposure. This results in a faster and stronger antibody response. If the antigen is the same as before, it's called a specific recall reaction. If it's different, it's a non-specific recall, which may lead to a temporary rise in antibody levels. Our company offers high-quality and cost-effective ELISA kits, available in 96T and 48T formats. Whether you're conducting research or clinical testing, we provide reliable solutions tailored to your needs. For more information or assistance, feel free to contact us via phone, QQ, or mobile number. Let us support your scientific progress!

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