Dylight fluorescent dyes are a new generation of high-performance labeling agents designed for advanced fluorescence-based applications. Compared to traditional fluorophores, Dylight dyes have smaller molecular weights, allowing for higher labeling density on macromolecules without compromising performance. This means more Dylight molecules can be attached to a single target, resulting in stronger fluorescence signals and improved specificity. Additionally, Dylight dyes exhibit superior photostability, making them resistant to photobleaching—unlike FITC, which often loses its signal quickly during imaging.
Dylight 649 is one of the most popular dyes in the Dylight series, offering strong fluorescence intensity and excellent optical tolerance. Abbkine provides DyLight 649 Red Fluorescent Secondary Antibodies that deliver high specificity and versatility, ideal for a wide range of immunoassay applications. Below is a selection of available products:
| Product Name | Item No. | Specification |
|----------------------------------------|---------------------|-------------------|
| Dylight 649, Goat Anti-Mouse IgG | A23610 | 100ul / 500ul |
| Dylight 649, Goat Anti-Rabbit IgG | A23620 | 100ul / 500ul |
| Dylight 649, Rabbit Anti-Goat IgG | A23630 | 100ul / 500ul |
| Dylight 649, Goat Anti-Rat IgG | A23640 | 100ul / 500ul |
*Figure 1: Labeling of human jejunum using an anti-PGP primary antibody and DyLight 649 (red, upper left) or Cy5 (red, upper right) secondary antibodies. DyLight 649 shows a denser red signal.*
*Figure 2: Cytokeratin 18 protein in human lung cancer A549 cells labeled with an anti-Cytokeratin 18 antibody and DyLight 649 anti-rabbit secondary antibody. The blue region represents Hoechst-stained nuclei.*
*Figure 3: Flow cytometry analysis of human peripheral blood lymphocytes using anti-CD3 and various fluorescently conjugated secondary antibodies. Green = DyLight 649, Yellow = APC, Blue = Cy5, and Red = Alexa Fluor 647. Note the slight background seen with Alexa Fluor 647.*
Compared to other red fluorescent dyes like Cy5 and Alexa Fluor 647, DyLight 649 offers brighter signals and lower background noise. It is an ideal choice for flow cytometry when working within the appropriate emission spectrum. For confocal microscopy applications requiring multi-color labeling, DyLight 649 is particularly advantageous due to its far-red emission properties.
Additionally, DyLight-labeled secondary antibodies are more cost-effective than traditional dyes such as FITC, Cy2, and Alexa 488. Here’s a comparison of excitation and emission wavelengths:
| Dye | Ex/Em (nm) | Alternative Dyes |
|-------------|------------|------------------------|
| DyLight 649 | 646/674 | Alexa Fluor 647, Cy5 |
As a leading supplier of immunological reagents, Abbkine China offers a comprehensive range of products, including tag antibodies, internal reference antibodies, primary and secondary antibodies, detection substrates, and related kits. Amy Jiejie, as the agent for Abbkine, ensures customers receive high-quality, cost-effective solutions tailored to their research needs.
If you're interested in DyLight 649 or any of our other fluorescent products, feel free to contact us at **400-6800-868** for more information. For full experimental support and product details, reach out to Amytech Co., Ltd.
Source: [DyLight 649 Red Fluorescent Secondary Antibody](http://)
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